Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
نویسندگان
چکیده
منابع مشابه
Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a challenge. We present a new approach to single reaction efficiency calculation, called Full Proces...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2011
ISSN: 1362-4962,0305-1048
DOI: 10.1093/nar/gkr775